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Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems

Received: 23 April 2018     Accepted: 21 May 2018     Published: 8 June 2018
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Abstract

To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.

Published in Journal of Diseases and Medicinal Plants (Volume 4, Issue 3)
DOI 10.11648/j.jdmp.20180403.11
Page(s) 59-68
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Salvia miltiorrhiza, Culture System, Growth Stages, Bioactive Components, Growth

References
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    Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. (2018). Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. Journal of Diseases and Medicinal Plants, 4(3), 59-68. https://doi.org/10.11648/j.jdmp.20180403.11

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    Lilan Lu; Shilong Fu; Jian Feng; Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J. Dis. Med. Plants 2018, 4(3), 59-68. doi: 10.11648/j.jdmp.20180403.11

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    AMA Style

    Lilan Lu, Shilong Fu, Jian Feng, Jianhe Wei. Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems. J Dis Med Plants. 2018;4(3):59-68. doi: 10.11648/j.jdmp.20180403.11

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  • @article{10.11648/j.jdmp.20180403.11,
      author = {Lilan Lu and Shilong Fu and Jian Feng and Jianhe Wei},
      title = {Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems},
      journal = {Journal of Diseases and Medicinal Plants},
      volume = {4},
      number = {3},
      pages = {59-68},
      doi = {10.11648/j.jdmp.20180403.11},
      url = {https://doi.org/10.11648/j.jdmp.20180403.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20180403.11},
      abstract = {To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Growth and Accumulation of Five Main Bioactive Components in the Roots of Salvia miltiorrhiza at Different Growth Stages and Using Different Culture Systems
    AU  - Lilan Lu
    AU  - Shilong Fu
    AU  - Jian Feng
    AU  - Jianhe Wei
    Y1  - 2018/06/08
    PY  - 2018
    N1  - https://doi.org/10.11648/j.jdmp.20180403.11
    DO  - 10.11648/j.jdmp.20180403.11
    T2  - Journal of Diseases and Medicinal Plants
    JF  - Journal of Diseases and Medicinal Plants
    JO  - Journal of Diseases and Medicinal Plants
    SP  - 59
    EP  - 68
    PB  - Science Publishing Group
    SN  - 2469-8210
    UR  - https://doi.org/10.11648/j.jdmp.20180403.11
    AB  - To Growth and accumulation of five main bioactive components in the roots of Salvia miltiorrhiza at different growth stages and using different culture systems. We analyzed growth parameters and the accumulation of selected bioactive components in Salvia miltiorrhiza that was grown in quartz sand-pot (hydroponic culture), soil-pot, and field culture systems at 3 growth stages (flower, root swelling, and mature). The highest bioactive compound concentrations (danshensu (DSS), 0.618 mg·g-1; salvianolic acid B (SAB), 52.5 mg·g-1; cryptotanshinone (CTS), 0.617 mg·g-1; tanshinoneⅡA (TSⅡA), 1.11 mg·g-1; and total tanshinone (TTS), 2.5 mg·g-1, at the mature stage) were present in the roots of plants grown in the hydroponic culture system. These concentrations were significantly higher than those of plants grown in the field system. The highest values for root parameters ( longest root length (LRL), 46.72 cm; largest root diameter (LRD), 14.68 mm; and the number of roots per plant (RN), 9.56), plant biomass (shoot dry weight (SDW), 18.9 g·plant-1; root dry weight (RDW), 19.6 g·plant-1, at the mature stage), and yield (DSS, 8.36 mg·plant-1; SAB, 657 mg·plant-1; CTS, 7.95 mg·plant-1; TSⅡA, 15.2 mg·plant-1; and TTS, 30.7 mg·plant-1, at the mature stage) were obtained from plants grown in the field system. Plants grown in the field culture system had significantly greater plant biomass and higher yields of bioactive compounds than plants grown in the quartz sand-pot (hydroponic culture) and soil-pot systems. Greenhouse hydroponic culture provides sufficient bioactive compound accumulation in the roots, but does not stimulate plant growth and root production. Therefore, the field system could greatly improve plant growth and root production in S. miltiorrhiza.
    VL  - 4
    IS  - 3
    ER  - 

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Author Information
  • Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China

  • Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China

  • Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China

  • Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Haikou, China

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